Samples were provided at a concentration well outside the acceptable range for the chip. First run failed because we did not check the concentrations first.
We ran the diagnostic checks on the bioanalyzer and it passed all checks.
Because of the limited volume provided, we diluted the samples 1:100 (1 ul sample in 99 ul TE) and nano dropped them for concentration via absorbance.
Sample ID | Date | Time | 260/280 | ng/ul |
e28822q | 9/17/20 | 1:13 PM | 1.15 | 2.8 |
e28823q | 9/17/20 | 1:14 PM | 1.84 | 6.43 |
e28844q | 9/17/20 | 1:15 PM | 2.79 | 2.5 |
e28866q | 9/17/20 | 1:15 PM | 1.71 | 4.53 |
e28822k | 9/17/20 | 1:16 PM | 1.78 | 72.02 |
e28823k | 9/17/20 | 1:17 PM | 1.89 | 76 |
e28844k | 9/17/20 | 1:18 PM | 1.98 | 22.45 |
e28866k | 9/17/20 | 1:19 PM | 1.88 | 70.99 |
e28822o | 9/17/20 | 1:19 PM | 1.73 | 17.22 |
e28823o | 9/17/20 | 1:20 PM | 1.78 | 15.42 |
e28844o | 9/17/20 | 1:21 PM | 2.1 | 6.52 |
e28866o | 9/17/20 | 1:21 PM | 1.93 | 15.8 |
The three samples that were still outside the range of 1-50 ng/ul were diluted 1:2 (98 ul sample plus 98 ul TE).
The diluted samples were then ran on the bioanalyzer on a DNA 1000 chip. DNA present was all on the high end or outside the measurable high end.
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