Page Comparison

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•  Pull Plates Dibner_1-95_Norm, Dibner_96-190_Norm, MP101, and MP102 and allow to Thaw
•  Tap plates to drop liquid, Spin to drop liquid
•  SpeedVac to dry samples
•  Resuspend Dibner_1-95_Norm and Dibner_96-190_Norm with 12 ul of ultra pure water
•  Resuspend MP101 and MP102 with 25 ul ultra pure water
•  Transfer the below samples to a new plate

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• Equilibrate Beads to room Temperature

• Add 24 ul of MagBeads to each well and 15 ul of replicate to same well of replicate

• Pipette mix up and down 10 times.

• Incubate at RT for 5 minutes

• Secure plate on magnet plate; incubate at RT for 5 minutes (until wells are clear)

• Remove 65 ul from each well; keep tips to left or right depending on the column to avoid bead pellet.

• Add 100 ul Fresh 80% EtOH to each well. Incubate 30 seconds. Remove 100 ul from each well

• Add 100 ul Fresh 80% EtOH to each well. Incubate 30 seconds. Remove 100 ul from each well

• Reaspirate from each well to assure maximum EtOH removal

• Allow plate to air dry for 7 minutes.

• Remove sample plate from magnet plate.

• Add 40 ul TE; pipette mix 10+ times. Incubate 2 minutes at RT.

• Place sample plate back on magnet for 5 minutes or until all wells are cleared.

• Transfer 40 ul to labeled transparent plate (Plate1 PCR1 MIDPlate1 MIDPlate2)

• Transfer 10 ul from transparent plate to “PCR2” plate with Mastermix already added.

• Label Plate1 PCR2 MIDPlate1 MIDPlate2

• Seal “PCR2”s with bubble strips and run on thermocycler 35GSAF2 program

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Temp C

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Cycles

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