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310-29-2022 We will be pooling 21-2022 Contamination has been detected in most blanks likely from an Alfalfa 16S/ITS project that bookended this one. Many plates will need to be rerun.

5-02-2022 Data have been processed and can be found in (/project/microbiome/data/seq/cu_29april22novaseq5) on Teton.

Data were returned to us 4-29-22. Report confusion has been cleared up. It involved using the index reads they ran in their calculations.

All samples have been pooled and were mailed for sequencing on Monday, 4-11-22. We normalized all preps based off absorbance readings using the improved Nimbus pooling normalization protocol, Normalization_Tracking_Adaptive . We will be normalizing to 10 ng/ul. This will leave , leaving samples below this as is. We will normalize normalized in 20 ul, unless the starting concentration is was above 100 ng/ul. We will normalize then normalized in 50 ul for those samples only. 2 ul from each sample was added to a new plate and then . The Nimbus program will handle this volume switch on its own.this plate was pooled into a column and the column was pooled into a 15 ml tube.