...
Pool 4+ libraries together in equal volumes and concentrate with SpeedVac leaving at least 7 ul
Prepare Hybridization MM:
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Temp C | Cycles | Time |
|---|---|---|
95* | 1X | 3:00 |
98 | 14X | 0:20 |
60 | 14X | 0:30 |
72 | 14X | 0:45 |
72 | 1X | 5:00 |
8 | 1X | 0:00 |
2nd Hybridization
Pool 4+ libraries together in equal volumesCombine both PCRs from above and concentrate to ~7 ul
Prepare Hybridization MM:
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ul per Rxn | Reagent | Thaw notes | Rxns | ul Needed |
|---|---|---|---|---|
Block O | ||||
Block C | ||||
Block X |
Add 7 ul of a library PCR pool to each Blocker Mix (LIB) and mix by pipette
Add LIBs to Cycler running:
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