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Customer’s Expectations
End deliverable is:
parsed fasta or fastqOTU table and parsed Fasta
Service | Unit | Price $ |
qPCR standards | Set of <73 reaction plates | 15 |
qPCR check | Reaction plate* | 3 |
AM 18S library prep (2 step) | Sample | 11 |
AMV4.5NF–AMDGR primer investment | Once, assuming 200 samples | 500 |
AMV4.5NF–AMDGR Assay Testing/Sequencing | Once, assuming 200 samples | 1078iSeq |
Shipping and Handling | 50 | |
MiSeq Sequencing | ~8M ~20M Reads | 7781860 |
bioinformatics | hour | 80 |
*reaction plates= (samples*assays*2)/48 rounded up
AM 18S Study reaction plates= (200184*1)/96=32
Prep Only:
1078+500+(200184*11)+(3*32)+(1*15)=$3,802623
Outside Pricing: 3802 3623*1.2=$4,562347.6
+ MiSeq Sequencing at ~40,000 reads/sample:
7781860+3623+380250= $4$5,580533
Outside Pricing: $5,496$6257
Prep, Sequencing, OTU Table:
Inside: 4580 5483 + (80*4)= $4,900$5853
Outside: 5496 6207 + (112*4)= $5,944$6705