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AM 18S expectations and deliverables

AM 18S expectations and deliverables

Bo Stevens

This page is meant to clarify expectations of all parties

GTL’s Expectations

  • Customer pays for primers

  • Customer pays for assay testing

Customer’s Expectations

  • End deliverable is:

    • OTU table and parsed Fasta

Service

Unit

Price $

qPCR standards

Set of <73 reaction plates

15

qPCR check

Reaction plate*

3

AM 18S library prep (2 step)

Sample

11

AMV4.5NF–AMDGR

primer investment

Once, assuming 200 samples

500

AMV4.5NF–AMDGR

Assay Testing/Sequencing

Once, assuming 200 samples

1078

Shipping and Handling

 

50

MiSeq Sequencing

~20M Reads

1860

bioinformatics

hour

80

*reaction plates= (samples*assays*2)/48 rounded up

AM 18S Study reaction plates= (184*1)/96=2

 

Prep Only: 

1078+500+(184*11)+(3*2)+(1*15)=$3,623

Outside Pricing: 3623*1.2=$4,347.6

 

 + MiSeq Sequencing:

1860+3623+50= $5,533

Outside Pricing: $6257

 

Prep, Sequencing, OTU Table:

Inside: 5483 + (80*4)= $5853

Outside: 6207 + (112*4)= $6705