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Plate Name

Average Result (nanomoles)

5AL1_16S_Col3

15.27

5AL2_16S_Col3

8.38

5AL3_16S_Col3

20.55

5AL4_16S_Col3

59.48

5AL5_16S_Col3

18.49

5AL6_16S_Col3

1.33

5AL7_16S_Col3

10.39

5AL8_16S_Col3

11.29

5AL1_ITS_Col3

68.89

Full qPCR results below:

 

View file
nameNS5_SET1.csv

qPCR NovaSeq5_Set2

  • Make 1:1000 dilutions of column 3 from the PCR plates by adding 1 ul to 999 ul TE in a deep well plate:

...

Plate Name

Average Result (nanomoles)

5AL2_ITS_Col3

23.49

5AL3_ITS_Col3

25.91

5AL4_ITS_Col3

28.89

5AL5_ITS_Col3

37.02

5AL6_ITS_Col3

18.74

5AL7_ITS_Col3

30.43

5AL8_ITS_Col3

14.8

5LVD1_16S_Col3

61.73

5LVD2_16S_Col3

57.63

Full qPCR results below:

View file
nameNS5_SET2.csv

qPCR NovaSeq5_Set3

  • Make 1:1000 dilutions of column 3 from the PCR plates by adding 1 ul to 999 ul TE in a deep well plate:

...

Plate Name

Average Result (nanomoles)

5LVD3_16S_Col3

60.49

5LVD4_16S_Col3

56.58

5LVD5_16S_Col3

59.58

5LVD1_ITS_Col3

52.72

5LVD2_ITS_Col3

25.43

5LVD3_ITS_Col3

39.91

5LVD4_ITS_Col3

38.61

5LVD5_ITS_Col3

21.75

5FB1_16S_Col3

66.78

Full qPCR results below:

View file
nameNS5_SET3.csv

qPCR NovaSeq5_Set4

  • Make 1:1000 dilutions of column 3 from the PCR plates by adding 1 ul to 999 ul TE in a deep well plate:

...

Plate Name

Average Result (nanomoles)

5FB2_16S_Col3

35.64

5FB3_16S_Col3

31.55

5FB4_16S_Col3

45.58

5FB5_16S_Col3

9.5

5FB1_ITS_Col3

19.46

5FB2_ITS_Col3

83.23

5FB3_ITS_Col3

59.86

5FB4_ITS_Col3

45.49

5FB5_ITS_Col3

16.69

Full qPCR results below:

View file
nameNS5_SET4.csv

qPCR NovaSeq5_Set5

  • Make 1:1000 dilutions of column 3 from the PCR plates by adding 1 ul to 999 ul TE in a deep well plate:

...

Plate Name

Average Result (nanomoles)

5DG1_16S_Col3

42

5DG2_16S_Col3

31.72

5DG3_16S_Col3

76.88

5DG4_16S_Col3

55.24

5DG5_16S_Col3

48.91

5DG1_ITS_Col3

37.74

5DG2_ITS_Col3

37.41

5DG3_ITS_Col3Col3

34.67

5DG4_ITS_Col3

17.29

Full qPCR results below:

View file
nameNS5_SET5.csv

qPCR NovaSeq5_Set6

  • Make 1:1000 dilutions of column 3 from the PCR plates by adding 1 ul to 999 ul TE in a deep well plate:

...

Plate Name

Average Result (nanomoles)

5DG5_ITS_Col3

27.22

5RD1_16S_Col1

2.79

5RD1_16S_Col2

0.573

5RD1_16S_Col3

2.64

5RD1_16S_Col4

8.07

5RD1_16S_Col5

10.31

5RD1_16S_Col6

4.6

5RD1_16S_Col7

13.52

5RD1_ITS_Col1

5.66

Full qPCR results below:

View file
nameNS5_SET6.csv

qPCR NovaSeq5_Set7

  • Make 1:1000 dilutions of column 3 from the PCR plates by adding 1 ul to 999 ul TE in a deep well plate:

...

Plate Name

Average Result (nanomoles)

5RD1_ITS_Col2

3.89

5RD1_ITS_Col3

5.49

5RD1_ITS_Col4

5.19

5RD1_ITS_Col5

9.37

5RD1_ITS_Col6

8.81

5RD1_ITS_Col7

16.43

Ha_Yoon_16S_ITS_Col1

19.16

Ha_Yoon_16S_ITS_Col6

18.35

 Ha_Yoon_16S_ITS_Col12

15.73

Full qPCR results below:

View file
nameNS5_SET7.csv