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Add 7 ul Master mix to each well of a new plate prepared as per the Amplicon MM protocol:
ul/rxn | Reagent |
|---|---|
3 | 5X KAPA HiFi HotStart PCR Buffer |
0.45 | 10M dNTPs |
0.3 | Kapa HiFi HotStart DNA Pol |
7.25 | HPLC H2O |
11 | Total Volume |
...
Transfer 40 ul to new plate.
Quantify via absorbance resulting plate.
Use Nimbus to normalize to 10ng/ul or less.
Pool resulting products.
Check Pool’s molar concentration via qPCR:
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