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Bo Stevens

Samples:

Design Info:

Paper detailing target and base primers

Fwd Primer Base (WANDA): CAGCCGCGGTAATTCCAGCT

Fwd Primer Base (AMV4.5NF): AAGCTCGTAGTTGAATTTCG

Rev Primer Base (AMDGR): CCCAACTATCCCTATTAATCA

200 sample multiplex required. Sticking to full columns for ease of handling, we will need 24 forward and 24 reverse primers for 576 possible combinations.

Design and sequences of 2-step sequencing primers.

Eurofins order form

Test Samples: Randomly selected good concentration (10ng/ul) normalized actual samples

Primer Dilution and arraying:

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