Below you can find a list of the sample plates used in the Alfalfa GBS sequencing, the EcoRI MID plates used, the pools created for PCR amplification, and the final pools created for sequencing.
GBS Plates | EcoR1 MID | PCR Pool | qPCR Quant |
ALF_PLT1_2017_GBS | EcoR1 Plate1 | Pool 1A | 1A: 107 nanomoles |
ALF_PLT2_2017_GBS | EcoR1 Plate2 | ||
ALF_PLT3_2017_GBS | EcoR1 Plate3 | ||
ALF_PLT4_2017_GBS | EcoR1 Plate4 | ||
ALF_PLT5_2017_GBS | EcoR1 Plate7 | Pool 3A | 3A: 259 nanomoles |
ALF_PLT6_2017_GBS | EcoR1 Plate8 | ||
ALF_PLT7_2017_GBS | EcoR1 Plate1 | ||
ALF_PLT8_2017_GBS | EcoR1 Plate2 | ||
ALF_PLT10_2017_GBS | EcoR1 Plate5 | Pool 2A | 2A: 96 nanomoles |
ALF_PLT11_2017_GBS | EcoR1 Plate6 | ||
ALF_PLT9_2017_GBS | EcoR1 Plate3 |
| 3C: 245 nanomoles |
ALF_PLT1_2020_GBS | EcoR1 Plate4 | ||
ALF_PLT2_2020_GBS | EcoR1 Plate5 | ||
ALF_PLT3_2020_GBS | EcoR1 Plate6 | ||
ALF_PLT4_2020_GBS | EcoR1 Plate7 | Pool 3E | 3E: 155 nanomoles |
ALF_PLT5_2020_GBS | EcoR1 Plate8 | ||
ALF_PLT6_2020_GBS | EcoR1 Plate1 | ||
ALF_PLT1_2020_END | EcoR1 Plate2 | ||
ALF_PLT2_2020_END | EcoR1 Plate3 | Pool 3G | 3G: 136 nanomoles |
ALF_PLT3_2020_END | EcoR1 Plate4 | ||
ALF_PLT4_2020_END | EcoR1 Plate5 | ||
ALF_PLT5_2020_END | EcoR1 Plate6 | ||
ALF_PLT6_2020_END | EcoR1 Plate7 | Pool 3I | 3I: 242 nanomoles |
ALF_PLT7_2020_END | EcoR1 Plate8 | ||
ALF_PLT8_2020_END | EcoR1 Plate1 |
Final Pools* | |
GBS Pool 1 | 3CD-3EF (50uL of each) |
GBS Pool 2 | 3AB-3GH (50uL of each) |
GBS Pool 3 | 1AB-2AB (100uL of 1AB, 50uL of 2AB) |
GBS Pool 4 | 3IJ (Combined using 150uL of 3I and 3J) |
*The final pools were then run on the Pippin Prep for size selection. For Pippin results click here.