We ran both diluted and neat versions of the four final pools through the Pippin Prep. Eluted product was in the 300-450bp range.
Bioanalyzer Results for Diluted GBS Pools
Gel:
EPG:
Bioanalyzer Results for Neat GBS Pools
Gel:
EPG:
qPCR Results for Diluted and Neat GBS Pools
GBS_Pool1_Diluted: 1.63 nanomoles
GBS_Pool2_Diluted: 0.85 nanomoles
GBS_Pool3_Diluted: 0.89 nanomoles
GBS_Pool4_Diluted: 2.74 nanomoles
GBS_Pool1_Neat: 12.73 nanomoles
GBS_Pool2_Neat: 10.6 nanomoles
GBS_Pool3_Neat: 9.91 nanomoles
GBS_Pool4_Neat: 8.64 nanomoles
To view the full result report, see document below: