Vortex and quick spin deep well plates.
Use the 50 ml Integra expandable spacing pipette to transfer 30 ul from each well into a transparent PCR plate. Label the plate as shown here.
If it is only a few shy of a full plate, add 30 ul TE to fill out the last column and treat as samples. (ie. Abby’s second plate)
Coligos added during extraction so do NOT add during sample processing.
Seal, vortex, and spin aliquot plate.
Make a new folder under DNA Quantification labeled “Alfalfa Buerkle Lab 16S/ITS”. Check concentration on Synergy HTX and save file on petalibrary as plate name. Add a circle around the asterisk when concentrations have been measured. Normalize plates on the Hamilton Nimbus to 10ng/uL.
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