Plant samples from 2 replicates of 50 individuals were planted in 19 common gardens across the USA. This will explore the interaction of plant species, microbiome, and climate. We will explore some cheaper extraction and PCR methods for library prep.
Extractions
Try the cheaper Machery-Nagel kits out
Organization
Naming Convention: 2021-UWEC-411-2
Year-Garden-Species-Block
Blocks should be 1 or 2, any other number is likely row
Add “R” before last number for all samples in a bag with numbers higher than 2: 2021-UWEC-321-R3
Deal with each outer bag together to check for errors
Each Garden should have 2 blocks
Each block should have one of each species ID
Each Block is arranged 5x10
Washing
Load sample in tube
Fill half way with Ultra Pure Water
Shake in Mixer Mill (or Vortex?)
Dump out Liquid
Continue from Step 2 once
Save both washes separately for ?half? of the samples