Purpose:
Interrogating small artificial bacterial communities suspended in matrix to support controlled environment hydroponic agriculture at time intervals
closely related species, 16S too similar
RNA polymerase β subunit requested target
https://pmc.ncbi.nlm.nih.gov/articles/PMC6372929/#sec15
16S-27F and 23S-2241R
Supplementary Table 1. Primer sequences for full-length 16S rRNA gene and rrn operon amplification | |||||
|---|---|---|---|---|---|
Oligo name | ONT Universal Tag | Sequence 5' to 3' | Amplicon | Ref. | |
16S-27F | TTTCTGTTGGTGCTGATATTGC | AGRGTTTGATYHTGGCTCAG | 16S, rrn | -28 | |
23S-2241R | ACTTGCCTGTCGCTCTATCTTC | ACCRCCCCAGTHAAACT | rrn | -28 | |
Fwd Primer to order: TTTCTGTTGGTGCTGATATTGCAGRGTTTGATYHTGGCTCAG
Rev Primer to order: ACTTGCCTGTCGCTCTATCTTCACCRCCCCAGTHAAACT
Barcoding 1700