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DNA Extraction:

DNA extraction from feces was performed with the DNA Stool Mini kit (Qiagen) according to the vendor's protocol with modifications as specified below. Digestion was conducted overnight at 56°C in a shaker 550 rpm. After two washing steps with the provided buffer, the DNA was sequentially recovered in 2 × 75 μl buffer AE and stored at −20°C.

https://doi.org/10.1002/ece3.3951

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