Do an annealing temperature gradient for PCR (Row D will be suggested and then do up and down temperature from there).
Row A - 6 plants, 6 stools copied down to each row (full plate) see setup below
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
A | P1 | P2 | P3 | P4 | P5 | P6 | S1 | S2 | S3 | S4 | S5 | S6 |
B | P1 | P2 | P3 | P4 | P5 | P6 | S1 | S2 | S3 | S4 | S5 | S6 |
C | P1 | P2 | P3 | P4 | P5 | P6 | S1 | S2 | S3 | S4 | S5 | S6 |
D | P1 | P2 | P3 | P4 | P5 | P6 | S1 | S2 | S3 | S4 | S5 | S6 |
E | P1 | P2 | P3 | P4 | P5 | P6 | S1 | S2 | S3 | S4 | S5 | S6 |
F | P1 | P2 | P3 | P4 | P5 | P6 | S1 | S2 | S3 | S4 | S5 | S6 |
G | P1 | P2 | P3 | P4 | P5 | P6 | S1 | S2 | S3 | S4 | S5 | S6 |
H | P1 | P2 | P3 | P4 | P5 | P6 | S1 | S2 | S3 | S4 | S5 | S6 |