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Do an annealing temperature gradient for PCR (Row D will be suggested and then do up and down temperature from there).

Row A - 6 plants, 6 stools copied down to each row (full plate) see setup below

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9

10

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12

A

P1

P2

P3

P4

P5

P6

S1

S2

S3

S4

S5

S6

B

P1

P2

P3

P4

P5

P6

S1

S2

S3

S4

S5

S6

C

P1

P2

P3

P4

P5

P6

S1

S2

S3

S4

S5

S6

D

P1

P2

P3

P4

P5

P6

S1

S2

S3

S4

S5

S6

E

P1

P2

P3

P4

P5

P6

S1

S2

S3

S4

S5

S6

F

P1

P2

P3

P4

P5

P6

S1

S2

S3

S4

S5

S6

G

P1

P2

P3

P4

P5

P6

S1

S2

S3

S4

S5

S6

H

P1

P2

P3

P4

P5

P6

S1

S2

S3

S4

S5

S6

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