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Received blood, blood spot, and quill tip samples from Liam Berigan; University of Maine on 3-23-2022. This is a sponsored research project through Josh Jahner consisting of extractions and GBS preps. |
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All sample types will be extracted using Qiagen’s DNeasy Blood and Tissue Kits. The feather/quill samples will be extracted as shown in the Quill Tip Protocol involving an at least 12 hour digestion. SMin Quill and QLB samples showed high contamination after extraction. These samples were cleaned up using Zymo’s onestep-pcr-inhibitor-removal-kit. After quantification, a significant number of the QLB and quill samples were lower than the recommended range. We evaporated these and resuspended them in 50 ul ultra pure water.
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Pool | Plate1 Col 1-6 | Plate1 Col 7-12 | Plate2 Col 1-6 | Plate2 Col 7-12 |
|---|---|---|---|---|
1Smin Pool A1 |
Pool | Plate1 Col 1-6 | Plate1 Col 12-7 | Plate2 Col 1-6 | Plate2 Col 12-7 |
|---|---|---|---|---|
1Smin Pool A2 |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
A | PLT1_Col1 PLT1_Col7 PLT2_Col1 PLT2_Col7 | PLT1_Col2 PLT1_Col8 PLT2_Col2 PLT2_Col8 | PLT1_Col3 PLT1_Col9 PLT2_Col3 PLT2_Col9 | PLT1_Col4 PLT1_Col10 PLT2_Col4 PLT2_Col10 | PLT1_Col5 PLT1_Col11 PLT2_Col5 PLT2_Col11 | PLT1_Col6 PLT1_Col12 PLT2_Col6 PLT2_Col12 | PLT1_Col1 PLT1_Col12 PLT2_Col1 PLT2_Col12 | PLT1_Col2 PLT1_Col11 PLT2_Col2 PLT2_Col11 | PLT1_Col3 PLT1_Col10 PLT2_Col3 PLT2_Col10 | PLT1_Col4 PLT1_Col9 PLT2_Col4 PLT2_Col9 | PLT1_Col5 PLT1_Col8 PLT2_Col5 PLT2_Col8 | PLT1_Col6 PLT1_Col7 PLT2_Col6 PLT2_Col7 |
B | ||||||||||||
C | ||||||||||||
D | ||||||||||||
E | ||||||||||||
F | ||||||||||||
G | ||||||||||||
H |
Pool | Plate3 Rows A-B | Plate3 Rows C-D | Plate3 Rows E-F | Plate3 Rows G-H |
|---|---|---|---|---|
PJDA |
Pool | Plate3 Rows A-B | Plate3 Rows D-C | Plate3 Rows E-F | Plate3 Rows H-G |
|---|---|---|---|---|
PJDB |
Pool | Plate3 | Plate3 | Plate3 | Plate3 |
|---|---|---|---|---|
Mix1 | A7, C7, E7, G7 | B7, D7, F7, H7 |
Pool | Plate3 | Plate3 | Plate3 | Plate3 |
|---|---|---|---|---|
Mix2 | A7-D7 | E7-H7 |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
A | PLT3_Row A, Row C, Row E, Row G | PLT3_Row B, Row D, Row F, Row H | PLT3_Row A, Row D, Row E, Row H | PLT3_Row B, Row C, Row F, Row G | PLT3_A7, C7, E7, G7 | |||||||
B | PLT3_B7, D7, F7, H7 | |||||||||||
C | PLT3_A7, B7, C7, D7 | |||||||||||
D | PLT3_E7, F7, G7, H7 | |||||||||||
E | ||||||||||||
F | ||||||||||||
G | ||||||||||||
H |
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Fill out the Shipping and Receiving domestic shipping form.
email unsigned pdf to shipping.request@uwyo.edu by noon
Print, sign, and attach to package
Fill out Sequencing submission form
Insert inside package and email to brian.woessner@cuanschutz.edu
Make sure labeled tubes and ice packs are secure and bring down to Berry Center Office