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Bo Stevens

This page is meant to clarify expectations of all parties

GTL’s Expectations

  • Customer pays for primers

  • Customer pays for assay testing

Customer’s Expectations

  • End deliverable is:

    • ready to run library pool

    • parsed fasta or fastq

    • otu table

  • next

Service

Unit

Price $

qPCR standards

Set of <73 reaction plates

15

qPCR check

Reaction plate*

3

AM 18S library prep (2 step)

Sample

11

AMV4.5NF–AMDGR

primer investment

Once, assuming 200 samples

500

AMV4.5NF–AMDGR

Assay Testing/Sequencing

Once, assuming 200 samples

1078

iSeq Sequencing

~8M Reads

778

bioinformatics

hour

80

*reaction plates= (samples*assays*2)/48 rounded up

AM 18S Study reaction plates= (200*1)/96=3

 

Prep Only

1078+500+(200*11)+(3*3)+(1*15)=$3,802

Outside Pricing: 3802*1.2=$4,562

 

 + Sequencing at ~40,000 reads/sample:

778+3802= $4,580

Outside Pricing: $5,496

 

Prep, Sequencing, OTU Table:

Inside: 4580 + (80*4)= $4,900

Outside: 5496 + (112*4)= $5,944

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