Synthgene and Coligo Information Sheet

Owned by Gregg Randolph
Last updated: May 22, 2020 by Joshua Harrison

16S Synthgene:

sequence: GTGCCAGCAGCCGCGGTAAGCCACAGATACGTACCGCTCATAACGCGAACCGAAGCGCAGTAGAAGTACTCCGTATCCTACCTCGGTCGTGGTTTAGGCTATCGACATCTTGCATGGGCTTCCCTAGTGAACTCTTGGGATGTATTAGATACCCTAGTAGTCC

Molecular Weight (https://www.bioinformatics.org/sms2/dna_mw.html)=50293.50 Daltons

50 pg = 30110868216750 daltons

50 pg of 16S SG = 30110868216750/50293.50=598699300.27490226371201 molecules=9.941634554334167e-16 moles. (https://www.convertunits.com/from/molecule/to/mole)

= 9.941634554334167e-7 nMoles

50 pg/ul = 9.941634554334167e-7 nMoles/ul

 

 

16Scoligos:

Arrived dried 4 nMoles of each.

Dilute to 9.941634554334167e-7 nM/ul

4/9.941634554334167e-7 = 4.02 e6 ul = 4.02 L