Genome Technologies Laboratory

This is the home of the Genome Technologies Laboratory, a shared University of Wyoming lab that supports analyses of genomes.  The lab is located in the Berry Center in Room 320, is staffed by @Gregg Randolph and @Shannon Harris, and supervised by @Alex Buerkle.  Please use the sidebar to the left to navigate the structure of this website.

Services:

  1. DNA library prep for Illumina sequencing

    1. 16S (bacterial barcoding), ITS(fungus), COI(animals), 18S(designed for arbuscular mycorhyzal fungus), and TRNL (herbivore diet barcoding) amplicon library preps with high levels of multiplexing

    2. ddRAD GBS for UWyo Associated clients only

    3. Other libraries preps for Illumina sequencing

  2. DNA extraction using Qiagen kits

  3. Consulting

  4. Oxford Nanopore MinIon prep and sequencing

We regularly prepare DNA sequencing libraries for Illumina instruments.  Principally we have our libraries sequenced elsewhere, including the Genomic Sequencing and Analysis Facility (GSAF) at the University of Texas; at a commercial provider, Psomagen in Maryland (formerly Macrogen); or at the Genomics Core of University of Colorado Anschutz Medical Campus.  For pilot or other small scale projects, we can sequence Illumina libraries on an iSeq instrument.  We have robotic liquid handling and other equipment for high throughput DNA extractions using Qiagen kits and DNA sequencing library preparation.

Information regarding the GTL 

 

Equipment in the lab includes

  • ABI 7500 Real Time PCR System (quantitative PCR)

  • Agilent 2100 Bioanalyzer (Fragment Analyzer)

  • Bio Rad C1000 Touch Thermocyclers

  • Biotek Synergy HTX (multimode plate reader)

  • Hamilton Microlab Nimbus (Automated Liquid Handling - Library Prep)

  • Illumina iSeq 100 (small project DNA sequencing)

  • Integra Biosciences Assist Plus (Automated Liquid Handling - Extraction)

  • Nanodrop ND-1000 (Spectrophotometer-protein and nucleic acid quantification)

  • Oxford Nanopore MinIon (A third generation sequencer for longer reads)

  • Savant Speedvac DNA 130 (Vacuum concentrator)

Pages for use by GTL staff and micro project users



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