This is the home of the Genome Technologies Laboratory, a shared University of Wyoming lab that supports analyses of genomes. The lab is located in the Berry Center in Room 320, is staffed by @Gregg Randolph and @Shannon Harris, and supervised by @Alex Buerkle. Please use the sidebar to the left to navigate the structure of this website.
DNA library prep for Illumina sequencing
16S (bacterial barcoding), ITS(fungus), COI(animals), 18S(designed for arbuscular mycorhyzal fungus), and TRNL (herbivore diet barcoding) amplicon library preps with high levels of multiplexing
ddRAD GBS for UWyo Associated clients only
Other libraries preps for Illumina sequencing
DNA extraction using Qiagen kits
Oxford Nanopore MinIon prep and sequencing
We regularly prepare DNA sequencing libraries for Illumina instruments. Principally we have our libraries sequenced elsewhere, including the Genomic Sequencing and Analysis Facility (GSAF) at the University of Texas; at a commercial provider, Psomagen in Maryland (formerly Macrogen); or at the Genomics Core of University of Colorado Anschutz Medical Campus. For pilot or other small scale projects, we can sequence Illumina libraries on an iSeq instrument. We have robotic liquid handling and other equipment for high throughput DNA extractions using Qiagen kits and DNA sequencing library preparation.