...
Quantify on plate reader
Normalize on nimbus
Add 2 ul DNA to plates with 96-channel
qPCR
...
BMT is estimated to be at a concentration of 5.4 nM.
Dilute first to 1 nM by adding 10 ul to 44 ul RSB
Dilute to 100 pM add 10 ul to 88 ul RSB and 2 ul PhiX.
Load 20 ul