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Version 1 Current »

  • Add 9 ul MM to 10 plates (only 4 will be used)

  • Add fwd primers with Nimbus

  • Add rev primers with 96-channel

  • Add 4 ul 0.01 pg/ul ISD mix to the last 6 columns of a new plate

  • Use repeat pipette to add 20 in matching order to the two halves of this plate

BT-1

BT-9

BT-17

BT-25

BT-33

BT-41

BT-1

BT-9

BT-17

BT-25

BT-33

BT-41

BT-2

BT-10

BT-18

BT-26

BT-34

BT-42

BT-2

BT-10

BT-18

BT-26

BT-34

BT-42

BT-3

BT-11

BT-19

BT-27

BT-35

BT-43

BT-3

BT-11

BT-19

BT-27

BT-35

BT-43

BT-4

BT-12

BT-20

BT-28

BT-36

B1

BT-4

BT-12

BT-20

BT-28

BT-36

B1

BT-5

BT-13

BT-21

BT-29

BT-37

MC

BT-5

BT-13

BT-21

BT-29

BT-37

MC

BT-6

BT-14

BT-22

BT-30

BT-38

MC

BT-6

BT-14

BT-22

BT-30

BT-38

MC

BT-7

BT-15

BT-23

BT-31

BT-39

TE

BT-7

BT-15

BT-23

BT-31

BT-39

TE

BT-8

BT-16

BT-24

BT-32

BT-40

TE

BT-8

BT-16

BT-24

BT-32

BT-40

TE

  • Quantify on plate reader

  • Normalize on nimbus

  • Add 2 ul DNA to plates with 96-channel

  • No labels