Last Test?
- Gregg Randolph
Add 9 ul MM to 10 plates (only 4 will be used)
Add fwd primers with Nimbus
Add rev primers with 96-channel
Add 4 ul 0.01 pg/ul ISD mix to the last 6 columns of a new plate
Use repeat pipette to add 20 in matching order to the two halves of this plate
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BT-1 | BT-9 | BT-17 | BT-25 | BT-33 | BT-41 | BT-1 | BT-9 | BT-17 | BT-25 | BT-33 | BT-41 |
BT-2 | BT-10 | BT-18 | BT-26 | BT-34 | BT-42 | BT-2 | BT-10 | BT-18 | BT-26 | BT-34 | BT-42 |
BT-3 | BT-11 | BT-19 | BT-27 | BT-35 | BT-43 | BT-3 | BT-11 | BT-19 | BT-27 | BT-35 | BT-43 |
BT-4 | BT-12 | BT-20 | BT-28 | BT-36 | B1 | BT-4 | BT-12 | BT-20 | BT-28 | BT-36 | B1 |
BT-5 | BT-13 | BT-21 | BT-29 | BT-37 | MC | BT-5 | BT-13 | BT-21 | BT-29 | BT-37 | MC |
BT-6 | BT-14 | BT-22 | BT-30 | BT-38 | MC | BT-6 | BT-14 | BT-22 | BT-30 | BT-38 | MC |
BT-7 | BT-15 | BT-23 | BT-31 | BT-39 | TE | BT-7 | BT-15 | BT-23 | BT-31 | BT-39 | TE |
BT-8 | BT-16 | BT-24 | BT-32 | BT-40 | TE | BT-8 | BT-16 | BT-24 | BT-32 | BT-40 | TE |
Quantify on plate reader
Normalize on nimbus
Add 2 ul DNA to plates with 96-channel
qPCR
BMT is estimated to be at a concentration of 5.4 nM.
Dilute first to 1 nM by adding 10 ul to 44 ul RSB
Dilute to 100 pM add 10 ul to 88 ul RSB and 2 ul PhiX.
Load 20 ul