This experiment is to verify these CO1 primers can be sequencedtest the latest extraction method, QIAamp Fast DNA Stool Mini Kit using the larger volume protocol. Primer Source Paper
Primer bases:
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Step | Temp C | Cycles | Time |
|---|---|---|---|
Denature | 95 | 1X | 10:00 |
Denature | 94 | 35X | 1:00 |
Annealing** (Row C) | 50 | 35X | 1:30 |
Extension/Elongation | 72 | 35X | 1:00 |
Final Extension | 72 | 1X | 10:00 |
Hold | 4 | 1X | 0:00 |
Run samples on TapeStation with D1000 kit:
Positive Control Butterfly (D1):
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