SOP for Dionex Ion Chromatography HPIC System

SOP for Dionex Ion Chromatography HPIC System Version 1.2

Approved by laboratory manager on 1-31-2022

For Running the Anion side

Log on to the Anion Computer
1. Username: Ion Chromatography
2. Password: EcologyBGC
Click on Chromeleon Software
Click on instrument tab
Select “Integrion_Anions” on the left (if it isn’t selected automatically)
(tabs at the top, left to right)
Sampler *do not touch*
Click on “Pump_ECD” and do the following:
1. Flow rate – 1.0mL/minute
  1. Type in gray boxes and hit the “enter” key
2. Turn On Eluent Generator
  1. 30.00mM concentration
3. Turn On General On
4. Turn on CR_TC Anions
5. Turn on Suppressor Anions
6. Column Temperature should be 30
7. When CD total is less than 1microS you can run
8. Everything should have a green light
Click “Take Control” at the top bar
1. If you cannot take control then someone else forgot to “Release Control”
Data Tab On lower left
1. Look for the folders “Integrion_Anions” on the Anion Computer and expand them using the small “+” sign
2. Locate your name and date
  1. If you do not have a folder yet, right click on the “Integrion_Anions” folder and create a folder with your name and date
3. Then locate the “Templates as of 2022” folder and expand it
4. Open the “Anion Sequences” folder using the “+” symbol
5. Copy over the sequence named “Anions Full Tray Run with calibration 11-3-2021” in the “Anion Sequence” folder and paste to your name folder that is under “Integrion_Anions” and rename the sequence to include the date of the run first then your name and whatever you want to name your sequence.
6. Example: Jake, needs to run 120 samples for his pharmaceuticals class. They copy the sequence name “Anions Full Tray Run with calibration 11-3-2021”, and put it under the folder “Jake 2022”.
  They rename the sequence to “1-12-2022 Jake Pharmaceuticals Tray 1”
7. To setup your schedule
8. The templates should start with a calibration, if you do not need the calibrations, you can delete them.
9. If you only need a partial tray delete, the number of samples you don’t need.
10. Your adjusted templates should start with a “blank” and a “CCV” or if you need calibrations, it will start with “Cal Std 1”
11. Following the “Blank” and “CCV” are 10 samples, numbered from 1 through 10 before the “CCV” and “Blank” need to be run again.
  1. Be sure to name your samples with your initials followed by a number/letter sequence, that would replace the place holder 1 through 10.
    1. For example, Jake Adams would be “JA01” instead of “1”.
12. At the end of your samples run a CCV A followed by a Blank
13. Double check to make sure you assigned a position to every sample to include the CCV and Blank(s)
14. Click “Save” at the top of the schedule
15. Loading Samples
  1. Push the carousel release button and wait for the arm to come up above the carousel tray.
  2. The carousel tray can now be moved freely and locate the position number that the sample will match up with.
16. Once everything is loaded and the schedule is completed and double checked for accuracy, go back to the “Instruments Tab” and then to the “Queue” tab of the Anion instrument.
17. Add your Anion sequence to the instrument by clicking “Add” and navigating to the “Instrument Data” folder and then to the “Integrion Anions” folder, then locate the folder with your name and select the correct sequence
18. Click “Ready Check” on the right side
19. If no errors, and the Ultra Purified Water containers on each instrument are full and the waste container is ¾ or less full, proceed to hit “Start” on the right side to start running your samples.
20. Click “Release Control” at the top bar if you are completed with your samples on the Anion instrument.

For running the Cation side

Log on to the Cation Computer
1. Username: Ion Chromatography
2. Password: EcologyBGC
Click on Chromeleon Software
Click on instrument tab
Select “Integrion_Cations” on the left (if it isn’t selected automatically)
(tabs at the top, left to right)
Sampler *do not touch*
Pump for Cations
1. Flow rate – 0.64mL/minute
2. Turn On Eluent Generator
  1. 35.00mM concentration
3. Turn On General On
4. Turn on CR_TC Cations
5. Turn on Suppressor Cations
6. When CD total is less than 1microS you can run
7. Column Temperature should be 40
8. Everything should have a green light
Click “Take Control” at the top bar
1. If you cannot take control then someone else forgot to “Release Control”
Data Tab On lower left
1. Look for the folders “Integrion_Cations” on the Cations Computer and expand them using the small “+” sign
2. Locate your name and date
  1. If you do not have a folder yet, right click on the “Integrion_Cations”, folder and create a folder with your name and date
3. Then locate the “Templates as of 2022” folder and expand it
4. Open the “Cation Sequences” folder using the + symbol
5. Copy over the sequence named “Cations-Full ASAP CCV Blank Template 11-3-2021” in the “Cation Sequence” folder to your name folder that is under “Integrion_Cation” and rename the sequence to include the date of the run first then your name and whatever you want to name your sequence.
6. Example: Jake, needs to run 120 samples for his pharmaceuticals class. They copy the sequence name “Cations-Full ASAP CCV Blank Template 11-3-2021”, and put it under the folder “Jake 2022”.
  They rename the sequence to “1-12-2022 Jake Pharmaceuticals Tray 1”
7. To setup your schedule
8. The templates should start with a calibration, if you do not need the calibrations, you can delete them.
9. If you only need a partial tray delete the number of samples you don’t need.
10. Your adjusted templates should start with a “blank” and a “CCV” or if you need calibrations, it will start with “Cal Std 1”
11. Following the “Blank” and “CCV” are 10 samples, numbered from 1 through 10 before the “CCV” and “Blank” need to be run again.
  1. Be sure to name your samples with your initials followed by a number/letter sequence, that would replace the place holder 1 through 10.
    1. For example, Jake Adams would be “JA01” instead of “1”.
12. At the end of your samples run a CCV A followed by a Blank
13. Double check to make sure you assigned a position to every sample to include the CCV and Blank(s)
14. Click “Save” at the top of the schedule
15. Loading Samples
  1. Push the carousel release button and wait for the arm to come up above the carousel tray.
  2. The carousel tray can now be moved freely and locate the position number that the sample will match up with.
16. Once everything is loaded and the schedule is completed and double checked for accuracy, go back to the “Instruments Tab” and then to the “Queue” tab of the Cation instrument.
17. Add your Cation sequence to the instrument by clicking “Add” and navigating to the “Instrument Data” folder and then to the “Integrion Cation” folder, then locate the folder with your name and select the correct sequence
18. Click “Ready Check” on the right side
19. If no errors, and the Ultra Purified Water containers on each instrument are full and the waste container is ¾ or less full, proceed to hit “Start” on the right side to start running your samples.
20. Click “Release Control” at the top bar if you are completed with your samples on the Cation instrument.