1SUCK bioinformatics

I split the file for faster processing:

mkdir -p /gscratch/grandol1/1SUCK/rawreads
cd /gscratch/grandol1/1SUCK/rawreads
unpigz --to-stdout /project/gtl/data/raw/1SUCK/rawreads/1SUCK_S1_R1_001.fastq.gz | split -l 16000000 -d --suffix-length=3 --additional-suffix=.fastq - 1SUCK_

Making 236 files

Demultiplexing

cd /gscratch/grandol1/1SUCK/rawreads/

Parse split files

/project/gtl/data/raw/1SUCK/demultiplex/run_parsebarcodes_onSplitInput.pl

Recombine by sample name and mid

/project/gtl/data/raw/1SUCK/demultiplex/run_splitFastq_gbs.sh

Genome Technologies Laboratory

1SUCK bioinformatics

Analytics

Demultiplexing

Related content