MasterMix

ul/rxn

Reagent

# of rxns

ul needed

ul/rxn

Reagent

# of rxns

ul needed

10

5X Phusion HF Buffer

7

70

1.5

10M dNTPs

7

10.5

1

Kapa HiFi HotStart DNA Pol

7

7

5 ul

5 uM F and R FlowCell Primers

7

35

17.5

HPLC H2O

7

122.5

35

Total Volume

7

1150

Â