Last Test?

Owned by Gregg Randolph

Last updated: May 02, 2023

1 min read

Add 9 ul MM to 10 plates (only 4 will be used)
Add fwd primers with Nimbus
Add rev primers with 96-channel
Add 4 ul 0.01 pg/ul ISD mix to the last 6 columns of a new plate
Use repeat pipette to add 20 in matching order to the two halves of this plate


BT-1	BT-9	BT-17	BT-25	BT-33	BT-41	BT-1	BT-9	BT-17	BT-25	BT-33	BT-41
BT-2	BT-10	BT-18	BT-26	BT-34	BT-42	BT-2	BT-10	BT-18	BT-26	BT-34	BT-42
BT-3	BT-11	BT-19	BT-27	BT-35	BT-43	BT-3	BT-11	BT-19	BT-27	BT-35	BT-43
BT-4	BT-12	BT-20	BT-28	BT-36	B1	BT-4	BT-12	BT-20	BT-28	BT-36	B1
BT-5	BT-13	BT-21	BT-29	BT-37	MC	BT-5	BT-13	BT-21	BT-29	BT-37	MC
BT-6	BT-14	BT-22	BT-30	BT-38	MC	BT-6	BT-14	BT-22	BT-30	BT-38	MC
BT-7	BT-15	BT-23	BT-31	BT-39	TE	BT-7	BT-15	BT-23	BT-31	BT-39	TE
BT-8	BT-16	BT-24	BT-32	BT-40	TE	BT-8	BT-16	BT-24	BT-32	BT-40	TE

Quantify on plate reader
Normalize on nimbus
Add 2 ul DNA to plates with 96-channel

qPCR

BMT is estimated to be at a concentration of 5.4 nM.

Dilute first to 1 nM by adding 10 ul to 44 ul RSB

Dilute to 100 pM add 10 ul to 88 ul RSB and 2 ul PhiX.

Load 20 ul