TRNL expectations and deliverables

 

This page is meant to clarify expectations of all parties

GTL’s Expectations

  • Customer pays for primers

  • Customer pays for assay testing

  • Samples delivered by end of May

Customer’s Expectations

  • End deliverable is:

    • ready to run library pool

    • raw data

    • parsed fasta or fastq

    • otu table

  • Data available by September

Service

Unit

Price $

Extraction

Sample

12

qPCR standards

Set of <73 reaction plates

15

qPCR check

Reaction plate*

2

Trnl only library prep

Sample

12

Trnl and 16S library prep

Sample

19

?Parasite locus? Library prep

Sample

10

ITS locus library prep

Sample

7

Trnl primer investment

Once, assuming 1000 samples

1000

Trnl Assay Testing

Once, assuming 1000 samples

2301

*reaction plates= (samples*assays*2)/48 rounded up

TRNL Study reaction plates= (500*2*2)/48 + (500*1*2)/48=63

 

Assuming 500 Fecal (trnl and 16S) and 500 plant (trnl only):

 

2301+1000+(500x19)+(500*12)+(63*2)+(1 * 15)+(1000*12)=$30,942