1Step Primer Arraying

Owned by Gregg Randolph
Dec 03, 2020
1 min read

  • Label hard shell PCR Plates in long16S0A1, long16S0B1, long16S0C1, . . . long16S0A2, long 16S0B2 . . . order

  • Run “LongPrimer_Prep_Tracker_FullyAdjustable” on Hamilton Nimbus

    • Start with Forward Primer Dilution

    • Follow with Reverse Primer Dilution

    • Then run Primer Arraying

    • Continue with Primer Arraying sub program using the correct number of plates that have been run

  • Use Benchsmart to add 102 ul TE to four labeled plates at a time in same order as above

  • Seal plates coming off the Nimbus with bubble seals and place in refrigerator 4