Amplified restriction fragments for genomic enrichment

Owned by Alex Buerkle
Dec 10, 2020
1 min read

This is a protocol that the Buerkle lab has commonly use for double-digest library prep of fragments for sequencing, to do genotyping by sequencing. The method does not have an acronym or name.

The lab method was first described in this paper, but has been iteratively improved since.

Parchman TL, Gompert Z, Mudge J, Schilkey F, Benkman CW, Buerkle CA (2012) Genome-wide association genetics of an adaptive trait in lodgepole pine. Molecular Ecology, 21, 2991–3005.

The protocol is hosted on Bitbucket for version control. Here is a pdf version.